Clinical Research Directory
Browse clinical research sites, groups, and studies.
Microbial Load After Apical Enlargement in Asymptomatic Teeth With Periapical Lesion
Sponsor: Istanbul Medipol University Hospital
Summary
The goal of this observational study is to define the role of apical shaping and irrigation activation on root canal cleanliness. The main questions it aims to answer are: * does the effectiveness of irrigation activation depends on apical shaping? * can a similar success be achieved by increasing apical shaping without irrigation activation Participants will \[describe the main tasks participants will be asked to do, treatments they'll be given and use bullets if it is more than 2 items\].
Official title: The Effect of Apical Enlargement on Microbial Load Before and After Passive Ultrasonic Activation in Asymptomatic Teeth Periapical Lesions
Key Details
Gender
All
Age Range
18 Years - 65 Years
Study Type
OBSERVATIONAL
Enrollment
24
Start Date
2024-02-24
Completion Date
2024-11-27
Last Updated
2026-11-04
Healthy Volunteers
Yes
Interventions
apical shaping size
24 patients will randomly be assigned to two groups according to the apical shaping size during root canal treatment: 25/.04 and 35/.04. Microbiological sampling of the teeth will be done with paper cones in three steps during the root canal treatment: 1. Sterility control samples (SR1) from the tooth surface after cavity preparation, 2. The first microbiological sample (S1) from the root canal after the initial file reaches the working length. 3. The second microbiological sample (S2) from the root canal after the shaping and irrigation of the root canal is finished. 4. The third microbiological sample (S3) from the root canal after the irrigation activation in the root canal is finished.The samples will be sent to the genetic analysis laboratory for further analysis in the cold chain using a Droplet Digital PCR.
Locations (1)
Istanbul Medipol University School of Dentistry
Istanbul, Turkey (Türkiye)