Clinical Research Directory

Inclusion Criteria: * Adult patients (≥18 years old) * Presence of at least 24 teeth * Presence of teeth #23-13 and #43-33 * Gingival Index (GI) ≤30% (Lindhe, 1981) * No interdental clinical attachment loss (CAL) at ≥2 non-adjacent teeth or buccal/oral CAL ≥3 mm with probing pocket depth (PPD) \>3 mm at ≥2 teeth (Tonetti et al., 2019) * No known allergy to chlorhexidine (CHX), hydrogen peroxide (H₂O₂), or cetylpyridinium chloride (CPC) * No fixed or removable orthodontic appliances or removable prostheses * No use of systemic antibiotics within the 3 months prior to the study * Non-smokers or light smokers (\<5 cigarettes/day) * Non-pregnant and non-lactating women * Absence of crowns, fixed partial dentures, or class III/IV/V composite restorations covering more than one-third of the crown, and no deep dental caries extending into dentine Exclusion Criteria: * Presented with signs of hypersensitivity, severe irritation, or discomfort during the experimental period * Were not compliant with the experimental protocol