Clinical Research Directory

* Applying a 15% hydrochloric acid gel (ICON-Etch; DMG; Hamburg, Germany®) for 120 s using the Icon® etch syringe included in the set, * followed by a thorough washing of the etchant for 30 s. and Dring the tooth using an air spray, * Once the enamel surface appeared chalky, Ethanol lesion desiccation (ICON-Dry; DMG®) will be performed for 30 s, * followed by drying using an air spray. * Using the Icon® infiltrant syringe Infiltration with a sufficient amount resin (ICON-Infiltrate; DMG®) of the lesion surface by massaging the tip of the syringe on the lesion in circular movements for 3 min. * Before drying, clean the remaining material and excess removal by Gauze or cotton swab and dental floss. * Light curing for 40 s with a light-cure device, * Then another round of infiltration will take place * One minute was provided for the material to infiltrate the pores, after which it was polymerized for 40 s.

* Isolation is extremely important because of the hydrochloric acid. Patients and clinician should both wear protective eyewear. * Isolate teeth to be treated and one tooth posterior on each side using rubber dam. * For additional protection, apply a bead of rubber dam sealer or light-cured resin barrier at the gingival margin beneath the dam. NOTE: For hypo-mineralization greater than 0.1-0.3mm use a fine-grit water-cooled tapered diamond bur, lightly sweep over the stained area for 5-10 seconds prior to applying Opalustre. * Apply approximately 1.0mm thick layer over the discolored area from Opalustre syringe with the White Mac tip. * Using a rubber prophy cup at a slow RPM (approximately 500 RPM), apply medium to heavy pressure for 60 seconds at a time. * Suction the paste from the teeth first, then rinse, evaluate and repeat as necessary. * Aesthetic improvement should be visualized on the wet surfaces. * After final rinse, remove rubber dam, and sealer from the teeth. Rinse

* The Antivet® abrasive solution (MDC DENTAL, Zapopan Jalisco, México) containing 21% hydrofluoric acid will be placed on the buccal surface with a cotton swab. * When the swab became pigmented, it will change to another clean swab with the abrasive solution. * The procedure would be repeated for 10 min. * When the process is finished, the excess solution will be cleaned using cotton, and calcium hydroxide as a neutralizing solution would be placed for 1 min with a micro brush. * In the end, rinsing with water for 30 s. * Apply MI varnish to the enamel.